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KMID : 1098420040120030203
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 3 p.203 ~ p.208
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Identification of Salidroside from Rhodiola sachalinensis A. Bor. and its Production through Cell Suspension Culture
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KIm Soo-Jung
Kim Kwang-Soo
Hwang Sung-Jin
Chon Sang-Uk
Kim Young-Ho
Ahn Jun-Cheul
Hwang Baik
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Abstract
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Salidroside was isolated and purified from R. sachalinensis A. Bor. roots. Purified salidroside was obtained from repeated silicagel column chromatography and preparative HPLC, and identified by ^1H-NMR,;^{13}C-NMR;and;^1H-^1H COSY spectra analyzer. Callus induction and cell suspension from R. sachalinensis leaf segments were established on 1/2MS solid medium and in 2B_5 liquid medium containing 0.5 mg/l NAA and 1 mg/l, BA in the dark condition, respectively. The contents of salidroside for suspension culture were ranging from 0.12% to 0.41% in comparison with 0.17% for natural roots.
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KEYWORD
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salidroside, callus induction, cell suspension culture, HPLC
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